IPTG 367-93-1

Application

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  Uses: Isopropyl-beta-D-thiogalactopyranoside [IPTG] is a very powerful inducer, which is not metabolized by bacteria and is very stable, so it is widely used in laboratories. IPTG is often used in cloning experiments that require the induction of β-galactosidase activity. It is often used in combination with X-Gal or Bluo-Gal for blue and white screening of recombinant bacterial colonies, which can induce the expression of the lac operon in E. coli. IPTG binds to the lacI repressor protein and changes its conformation to play a role, preventing the inhibition of the β-galactosidase encoding gene lacZ.

  Applicable to: E. coli induced expression, enzyme protein induced expression, gene induced expression, biological fermentation, usually used with X-GAL [CAS: 7240-90-6] for the screening of blue and white colonies. When IPTG is used for inducing expression experiments, the products usually matched together are X-GAL, SDS (sodium dodecyl sulfate), odor phenol blue, DTT, glycerin, PMSF, etc.